diff --git a/telegram/_poll.py b/telegram/_poll.py
index ce2c41c7c..9025315a8 100644
--- a/telegram/_poll.py
+++ b/telegram/_poll.py
@@ -29,8 +29,6 @@ from telegram._utils import enum
 from telegram._utils.argumentparsing import parse_sequence_arg
 from telegram._utils.datetime import extract_tzinfo_from_defaults, from_timestamp
 from telegram._utils.types import JSONDict
-from telegram._utils.warnings import warn
-from telegram.warnings import PTBDeprecationWarning
 
 if TYPE_CHECKING:
     from telegram import Bot
@@ -89,9 +87,11 @@ class PollAnswer(TelegramObject):
 
     .. versionchanged:: 20.5
         The order of :paramref:`option_ids` and :paramref:`user` is changed in
-        20.5 as the latter one became optional. We currently provide
-        backward compatibility for this but it will be removed in the future.
-        Please update your code to use the new order.
+        20.5 as the latter one became optional.
+
+    .. versionchanged:: NEXT.VERSION
+       Backward compatiblity for changed order of :paramref:`option_ids` and :paramref:`user`
+       was removed.
 
     Args:
         poll_id (:obj:`str`): Unique poll identifier.
@@ -145,21 +145,8 @@ class PollAnswer(TelegramObject):
         super().__init__(api_kwargs=api_kwargs)
         self.poll_id: str = poll_id
         self.voter_chat: Optional[Chat] = voter_chat
-
-        if isinstance(option_ids, User) or isinstance(user, tuple):
-            warn(
-                "From v20.5 the order of `option_ids` and `user` is changed as the latter one"
-                " became optional. Please update your code to use the new order.",
-                category=PTBDeprecationWarning,
-                stacklevel=2,
-            )
-            self.option_ids: Tuple[int, ...] = parse_sequence_arg(user)
-            self.user: Optional[User] = option_ids
-        else:
-            self.option_ids: Tuple[int, ...] = parse_sequence_arg(  # type: ignore[no-redef]
-                option_ids
-            )
-            self.user: Optional[User] = user  # type: ignore[no-redef]
+        self.option_ids: Tuple[int, ...] = parse_sequence_arg(option_ids)
+        self.user: Optional[User] = user
 
         self._id_attrs = (
             self.poll_id,
diff --git a/tests/test_poll.py b/tests/test_poll.py
index ab6dfea61..09133e908 100644
--- a/tests/test_poll.py
+++ b/tests/test_poll.py
@@ -22,7 +22,6 @@ import pytest
 from telegram import Chat, MessageEntity, Poll, PollAnswer, PollOption, User
 from telegram._utils.datetime import UTC, to_timestamp
 from telegram.constants import PollType
-from telegram.warnings import PTBDeprecationWarning
 from tests.auxil.slots import mro_slots
 
 
@@ -144,19 +143,6 @@ class TestPollAnswerWithoutRequest(TestPollAnswerBase):
         assert a != f
         assert hash(a) != hash(f)
 
-    def test_order_warning(self, recwarn):
-        expected_warning = (
-            "From v20.5 the order of `option_ids` and `user` is changed as the latter one"
-            " became optional. Please update your code to use the new order."
-        )
-        PollAnswer(123, [2], self.user, self.voter_chat)
-        assert len(recwarn) == 0
-        PollAnswer(123, self.user, [2], self.voter_chat)
-        assert len(recwarn) == 1
-        assert str(recwarn[0].message) == expected_warning
-        assert recwarn[0].category is PTBDeprecationWarning
-        assert recwarn[0].filename == __file__, "wrong stacklevel"
-
 
 @pytest.fixture(scope="module")
 def poll():